{"data":{"id":"us/27-cfr-21.99","jurisdiction":"us","citation":"27 CFR 21.99","heading":"Brucine alkaloid.","body":"(a) Identification test. Add a few drops of concentrated nitric acid to about 10 mg of brucine alkaloid. A vivid red color is produced. Dilute the red solution with a few drops of water and add a few drops of freshly made dilute stannous chloride solution. A reddish purple (violet) color is produced.\n(b) Melting point. 178 °±1 °C. Dry the alkaloid in an oven for one hour at 100 °C., increase the temperature to 110° and dry to a constant weight before taking melting point.\nNote.\nBrucine alkaloid tetrahydrate melts at 105 °C. while the anhydrous form melts at 178 °C.\n(c) Strychnine test. Brucine alkaloid shall be free of strychnine when tested by the method listed under Brucine Sulfate, N.F. IX.\nNote.\nIf the brucine contains as much as 0.05 percent strychnine, a clear distinctive violet color, characteristic of strychnine, will be obtained.\n(d) Sulfate test. No white precipitate is formed that is not dissolved by hydrochloric acid when several drops of a 1 N barium chloride solution are added to 10 mL of a solution of the alkaloid.","path":["Title 27—Alcohol, Tobacco Products and Firearms","CHAPTER I—ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY","SUBCHAPTER A—ALCOHOL","PART 21—FORMULAS FOR DENATURED ALCOHOL AND RUM","Subpart E—Specifications for Denaturants"],"source_url":"https://www.ecfr.gov/api/versioner/v1/full/2026-08-25/title-27.xml","current_through":"2026-08-25","vintage":"","retrieved_at":"2026-08-27T02:25:11Z","sha256":"eee4aa0065802be9088dbab49347d3c06151635a399607230d52b9502dbc0f18","source_id":"us-cfr","stale":true,"prev":"us/27-cfr-21.97-21.98","next":"us/27-cfr-21.100"},"notice":"GroundRules: Original legal text. Not legal advice."}