{"data":{"id":"us/9-cfr-113.32","jurisdiction":"us","citation":"9 CFR 113.32","heading":"Detection of Brucella contamination.","body":"The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product.\n(a) One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO2 atmosphere at a temperature of 35-37 °C for at least 7 days.\n(b) If colonies are identified as Brucella, the biological product is unsatisfactory.\n(c) If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either\n(1) The biological product shall be regarded as unsatisfactory and destroyed; or\n(2) Further subculture or other procedures shall be carried out until a positive identification can be made.","path":["Title 9—Animals and Animal Products","CHAPTER I—ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE","SUBCHAPTER E—VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS","PART 113—STANDARD REQUIREMENTS"],"source_url":"https://www.ecfr.gov/api/versioner/v1/full/2026-08-25/title-9.xml","current_through":"2026-08-25","vintage":"","retrieved_at":"2026-08-27T02:24:03Z","sha256":"77922db176382ccb4735216826bb14bf99a6ab0bb939b9edac7398b4a60c1abb","source_id":"us-cfr","stale":true,"prev":"us/9-cfr-113.31","next":"us/9-cfr-113.33"},"notice":"GroundRules: Original legal text. Not legal advice."}